CDR Annotation

1 简介

CDR Annotation是抗体编号和注释模块，用于对抗体序列的可变区（Fv）进行编号，精确标注出其框架区（Framework Region, FWR）和互补决定区（Complementarity Determining Region, CDR）的具体位置， 支持IMGT²、Kabat³、Chothia⁴、Martin⁵、AHo⁶、Wolfguy抗体编号方案。若一次输入多序列，还能通过查看序列可视化图和氨基酸频率图来分析序列的差异和保守性。

2 参数说明

• Antibody Sequences: 抗体氨基酸序列，支持多序列。
• Numbering Scheme: 抗体编号方案，支持IMGT、Kabat、Chothia、Martin、AHo、Wolfguy。

3 结果说明

Table 包含以下字段：

字段名称	说明
Chain Type	最显著对齐的链类型
Scheme	编号序列的编号方案
FWR1 AA	序列的 FWR1 区氨基酸
CDR1 AA	序列的 CDR1 区氨基酸
FWR2 AA	序列的 FWR2 区氨基酸
CDR2 AA	序列的 CDR2 区氨基酸
FWR3 AA	序列的 FWR3 区氨基酸
CDR3 AA	序列的 CDR3 区氨基酸
FWR4 AA	序列的 FWR4 区氨基酸

Chart: 对编号和注释后的序列可视化，CDR1、CDR2、CDR3 氨基酸频率LOGO图。

Figure 1. CDR Annotation 示例的可视化图

4 参考文献

[1] James Dunbar, Charlotte M. Deane, ANARCI: antigen receptor numbering and receptor classification, Bioinformatics, Volume 32, Issue 2, January 2016, Pages 298–300. https://doi.org/10.1093/bioinformatics/btv552
[2] Lefranc, M. P., & Lefranc, G. (2001). The IMGT unique numbering for immunoglobulin and T cell receptor variables and constant domains. Immunology Today, 22(8), 373-376. https://doi.org/10.1016/s0145-305x(02)00039-3
[3] Kabat, E. A., Wu, T. T., & Bilofsky, H. (1976). Unweighted groupings of amino acid residues in immunoglobulin variable and constant regions. In Kabat, E. A., & Mayer, M. M. (Eds.), Structural Concepts in Immunology and Immunochemistry (2nd ed., Vol. 1, pp. 1-66). Holt, Rinehart and Winston, New York.
[4] Chothia, C., & Lesk, A. M. (1987). Canonical structures for the hypervariable regions of immunoglobulins. Journal of Molecular Biology, 196(2), 901-917. https://doi.org/10.1016/0022-2836(87)90412-8
[5] Martin, A. C., & Thornton, J. M. (1996). Structural families in loops of homologous proteins: automatic classification, modelling and application to antibodies. Journal of Molecular Biology, 263(4), 800-815. https://doi.org/10.1006/jmbi.1996.0617
[6] Honegger, A., & Plückthun, A. (2001). Yet another numbering scheme for immunoglobulin variable domains: an automatic modeling and analysis tool. Journal of Molecular Biology, 309(3), 657-670. https://doi.org/10.1006/jmbi.2001.4662